Biology Results for 2nd July

Biology Results for 2^nd July; 2007

Experiment 1: Spectrophotometric analysis---Determination of the concentration of an unknown solution of biological dye Methylene Blue from a standard curve.

Title: Spectrophotometer analysis; Determination of the concentration of an unknown solution.

Professor Uddin’s Results:

Concentrations %	Absorption
0.0001	.015
0.001	.011
0.01	.029
0.1	.183
1	.454
unknown	.164

Students’ Results:

Group 1 Results		Group 2 Results	(This one is not good)
Concentrations %	Absorption	Concentrations %	Absorption
0.0001	.010	0.0001	.002
0.001	.024	0.001	.026
0.01	.026	0.01	.166
0.1	.231	0.1	.432
1	.516	1	.465
unknown	.130	unknown	.047

To determine the concentration of an unknown protein from Standard curve for Bovine Serum Albumin Protein
Spectrophotometric Determination & Protein Concentrations
Protein Concentrations(mg/ml)	Absorption
Protein Concentrations(mg/ml)	group I	group II	group III	average
0.1	1.01	1.03	1.05
0.2	1.03	1.04	1.07
0.3	1.07	1.08	1.10
0.4	1.20	1.09	1.27
0.5	1.36	1.37	1.42
0.6	1.44	1.44	1.48
0.7	1.48	1.48	1.50
0.8	1.56	1.56	1.58
1.0	1.90	1.90	1.95
unknown	1.08	1.08	1.08